FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838982
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10530.1255092481125936No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.029.6202031
GTATCAA22850.028.1665081
GTGGTAT5200.026.446611
TGGTATC5050.025.584492
CCGCGTG550.001228954624.99065224
TATCAAC26850.023.8551352
CAACGCA27300.023.4591245
TCAACGC27400.023.3735034
AACGCAG27300.023.256376
ATCAACG27700.023.0211333
ACGCAGA27950.022.7155257
CGCAGAG28300.022.4345918
AGAGTAC28100.022.30482511
CAGAGTA28850.021.72497610
TACGGGT4450.021.63144115
TGCCGAC3600.021.39874855
CTCGGTA650.003249429621.1421558
GCAGAGT30300.021.1364369
AGTACGG30900.019.93732813
GAGTACG31450.019.66672712
Produced by FastQC (version 0.11.5)