FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984190
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11950.030.1495821
TGGTATC11500.029.4049842
GTATCAA53250.028.458361
GGTATCA26650.026.8320621
TATCAAC60800.024.8246082
TCAACGC61850.024.1309974
ATCAACG62800.023.7245833
CAACGCA63500.023.5899475
AGAGTAC60600.023.09534811
AACGCAG64800.023.074866
ACGCAGA67450.022.1688447
CAGAGTA65200.021.75838910
CGCAGAG68950.021.6871098
AGTACGG68650.020.07077613
GCAGAGT76150.019.6034569
GAGTACG72000.019.13547912
TACGGGG31000.017.65506415
TACGCGA1101.8675727E-417.49873229
GTACGGG79650.017.22985814
GTATTAG4050.016.9770761
Produced by FastQC (version 0.11.5)