FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393010
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.31153233793423346No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52720.22030831463303538No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35090.14663540896193497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT21200.048.519151
TGGTATC23200.044.1458742
GTATCAA78800.042.018921
TATCAAC90200.036.607962
TCAACGC92100.035.712834
ATCAACG93150.035.355893
CAACGCA93900.035.0282365
GGTATCA40700.034.9851761
AACGCAG96550.034.066826
ACGCAGA105950.031.122647
CGCAGAG108150.030.4508868
GCAGAGT119600.027.6777679
CAGAGTA77250.023.68121310-11
AGAGTAC72700.022.00695210-11
GAGTACG86000.021.22304212-13
AGTACGG79100.020.11954912-13
GTACGGG90850.019.8096114-15
CCGGTCG1801.3540906E-518.8860999
TACGGGG36500.017.17301614-15
ACGGGAT17000.017.12318616-17
Produced by FastQC (version 0.11.5)