FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2393010
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT15450.032.050541
TGGTATC16100.030.5697232
GTATCAA70500.028.9538381
TATCAAC79750.025.8582
GGTATCA33550.025.501121
TCAACGC82600.024.5601924
ATCAACG84000.024.282263
CAACGCA84650.024.162275
AGAGTAC81150.023.98933411
AACGCAG85000.023.9657526
CAGAGTA84800.022.95533410
ACGCAGA89300.022.8723587
CGCAGAG90450.022.5207718
AGTACGG88650.021.59073813
TACGGGT9550.021.02290715
GCAGAGT97950.020.8831759
GAGTACG93100.020.52791612
TACGGGG39200.019.57453515
GTACGGG99150.019.1386614
ACGGGAT17250.018.81335316
Produced by FastQC (version 0.11.5)