FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207826
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68010.3080405792847806No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.2151437658583602No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.14335368819825475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22800.049.222661
TGGTATC22950.049.0717162
GTATCAA79100.042.0805361
TATCAAC89050.037.081322
ATCAACG92300.035.8200653
TCAACGC93550.035.3414424
CAACGCA94100.035.180045
AACGCAG98200.033.7112126
GGTATCA40400.032.4089781
CGCAGAG107850.030.733578
ACGCAGA109300.030.3251677
GCAGAGT113500.029.2785459
CAGAGTA74050.024.70510510-11
AGAGTAC72150.021.9690410-11
GAGTACG83850.021.53894612-13
GTACGGG86200.020.7050214-15
AGTACGG76950.020.48820312-13
ACGGGAT15150.018.23179616-17
TACGGGG33050.017.74358214-15
CCGTCTT15250.017.69787684-85
Produced by FastQC (version 0.11.5)