FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222648
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49870.22437201032282214No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34500.15522025979822265No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24550.11045383704482221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC19450.045.8917122
GTGGTAT19650.045.6501731
GTATCAA65500.041.4745861
GGTATCA34000.036.636271
TATCAAC74200.036.3751142
TCAACGC76650.035.4833154
ATCAACG77850.034.827193
CAACGCA78300.034.6270375
AACGCAG80700.033.860546
ACGCAGA86700.031.5655547
CGCAGAG88200.030.8359998
GCAGAGT99400.027.3621379
CAGAGTA65300.024.3399510-11
AGAGTAC61650.021.74841310-11
GAGTACG76900.020.64098512-13
GTACGGG77100.020.3118414-15
AGTACGG67900.019.74675612-13
ACGGGAT12750.019.66519416-17
CCGTGCG1306.2361313E-419.6139149
TACGGGG32500.017.91444614-15
Produced by FastQC (version 0.11.5)