FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2222648
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25560.11499796638963974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13800.032.8899651
TGGTATC13950.032.1252942
GTATCAA60400.031.151411
TATCAAC67650.027.7985442
TCAACGC70350.026.4894084
CAACGCA71250.026.2711285
ATCAACG72350.025.7974623
AACGCAG73200.025.686246
AGAGTAC70150.025.51736811
ACGCAGA75000.025.069777
CGCAGAG75450.024.8479448
CAGAGTA73650.024.37775410
GGTATCA26250.023.8926351
AGTACGG75550.023.3701413
GCAGAGT82550.022.6795489
GAGTACG80450.022.1156612
TACGGGG34000.021.19302215
GTACGGG83750.020.81925814
ACGGGAT15950.019.48445516
CGGGATT5600.019.15345417
Produced by FastQC (version 0.11.5)