FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827466
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29150.15951049157686106No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.10763538145169321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14850.044.361012
GTATCAA53850.043.0172541
GTGGTAT15850.042.101951
TATCAAC60200.038.0529632
GGTATCA27050.037.7116741
TCAACGC63300.035.9188584
ATCAACG63950.035.6866843
CAACGCA64100.035.669475
AACGCAG66200.034.6663556
ACGCAGA70600.032.4438747
CGCAGAG70600.032.3234868
GCAGAGT78750.029.0861959
CAGAGTA59100.024.37591410-11
AGAGTAC56650.021.04173910-11
GAGTACG69250.020.74175812-13
ACGGGAT12150.020.63594616-17
GTACGGG71950.019.8748114-15
TCTGTCG3401.0913936E-1118.7482628
AGTACGG63800.018.583712-13
CCGTCTT19000.018.45425684-85
Produced by FastQC (version 0.11.5)