FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830740
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29800.16277570818357603No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11000.034.2567061
TGGTATC10800.033.8594172
GTATCAA56750.029.710661
GGTATCA26300.029.387881
TATCAAC64100.026.6798842
TCAACGC66750.025.7805164
ATCAACG66850.025.7419513
CAACGCA67450.025.5129645
AACGCAG68850.025.1139626
ACGCAGA71050.024.2596117
AGAGTAC65500.024.22331411
CGCAGAG73050.023.6336988
CAGAGTA67800.023.51875110
TCGACGT2150.021.74606515
GCAGAGT79600.021.5853569
AGTACGG71400.021.56990213
GAGTACG75500.020.6148612
TACGGGG28850.020.49570515
CGACGTT2200.020.0017316
GTACGGG78450.019.38610514
Produced by FastQC (version 0.11.5)