FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036315
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35820.1759059870403155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14050.032.8789672
GTGGTAT15400.031.4467621
GTATCAA61300.027.471031
GGTATCA31800.026.3045231
TATCAAC68900.024.4639422
ATCAACG71600.023.4972443
TCAACGC71450.023.3524764
CAACGCA72150.023.090085
AGAGTAC69550.022.81258611
AACGCAG74100.022.669066
ACGCAGA75850.022.1465877
CAGAGTA71900.022.10413210
CGCAGAG76250.021.9588348
CACGCGT903.9964038E-521.38970232
AGTACGG76250.020.44995713
GCAGAGT82500.020.3967679
GAGTACG79600.019.76060112
ACGCGTA1008.983018E-519.25262333
TATACCG1009.015268E-519.2436395
TACGGGG31300.018.01186615
Produced by FastQC (version 0.11.5)