FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383906
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.2800045667841602No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.18324944035216265No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.11771030691390888No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT12950.051.855221
TGGTATC14150.047.1521262
GTATCAA48900.042.4146731
TATCAAC55350.037.2376442
ATCAACG56350.036.197123
TCAACGC56850.035.8787654
CAACGCA57950.035.1977165
GGTATCA26200.033.903941
AACGCAG60600.033.6585466
ACGCAGA65000.031.510877
CGCAGAG66100.031.0518938
GCAGAGT74450.027.797569
CAGAGTA47400.025.32692710-11
GGGTACG1802.1858796E-823.6119941
AGAGTAC45000.021.8615510-11
GAGTACG54350.021.85368512-13
GTACGAC1203.6355478E-421.2469563
ACGGGAT9900.019.74464616-17
GTACGGG59800.019.54116214-15
AGTACGG51150.019.10841212-13
Produced by FastQC (version 0.11.5)