FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037828
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13880.1337408510851509No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6550.034.4425161
TGGTATC6350.033.3401152
CGGACAA350.004096497331.433250
GTATCAA33200.029.3350941
GGTATCA15400.028.2266061
TATCAAC36800.026.2246322
ATCAACG37600.025.5861383
TCAACGC37800.025.3780444
GGTTACG550.001223356225.0109181
TAACGGC550.001225421625.00368336
CAACGCA39150.024.570785
AGAGTAC38500.024.50006511
AACGCAG40300.023.8719276
CGCAGAG40250.023.8332948
CAGAGTA39950.023.74392110
ACGCAGA41050.023.368827
GCAGAGT43000.022.3144479
AGTACGG42700.022.02900513
ACGGGTC1558.9858077E-1021.29345916
TACGGGG17800.020.85981415
Produced by FastQC (version 0.11.5)