FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059354
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.1349879266043268No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.1010049520745662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31750.043.917561
TGGTATC8100.039.3570752
GGTATCA15700.038.7208141
TATCAAC36950.037.7316672
GTGGTAT8600.037.5684471
TCAACGC38600.036.3321574
CAACGCA38700.036.2382745
ATCAACG38850.036.207753
AACGCAG40800.034.3730746
CGCAGAG43050.032.374558
ACGCAGA43400.032.309287
GCAGAGT46700.029.8456159
GTACGAC905.1567193E-528.3317433
CTAGACT1606.2500476E-926.561014
CAGAGTA35650.024.5545110-11
CGTCAGA2451.8189894E-1224.2843535
GTATAGG2254.110916E-1022.6728861
AGAGTAC34400.022.54384410-11
GAGTACG39600.021.94533512-13
CCGTCTT10750.021.7465684-85
Produced by FastQC (version 0.11.5)