FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125932
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.19139699377937566No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.14325909557593178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10900.044.844652
GTGGTAT11350.044.576521
GTATCAA36500.040.7690161
TATCAAC40850.036.938192
TCAACGC42250.035.308654
ATCAACG43500.034.4894453
CAACGCA43350.034.41275
GGTATCA19100.034.057531
AACGCAG44800.033.393766
ACGCAGA47550.031.3689127
CGCAGAG47950.031.1072338
GCAGAGT52100.028.9556649
CAGAGTA37100.025.02766810-11
GAGTACG40900.022.23480612-13
AGAGTAC34600.021.30915510-11
CGTCAGA2701.8007995E-1020.4635185
GTACGGG44850.019.99230414-15
ACGGGAT8150.019.55314316-17
AGTACGG39100.018.80235312-13
ATTACGC1150.00708156918.4787643
Produced by FastQC (version 0.11.5)