FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125932
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14280.12682826316331713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34950.031.8884091
GTGGTAT7300.031.2881741
TGGTATC7600.029.6686082
TATCAAC39750.027.60152
TCAACGC40300.027.2187584
AGAGTAC39550.027.04461311
CAACGCA41000.026.9552065
ATCAACG41050.026.7238353
CAGAGTA41300.026.36352310
GGTATCA14900.026.2256981
AACGCAG42400.026.1300146
ACGCAGA43450.025.4985647
CGCAGAG43850.025.2659668
AGTACGG44550.023.70385613
GCAGAGT47000.023.6321539
GAGTACG45700.023.40616812
TACGGGT4400.023.12617715
CCGATCG752.8733452E-421.99916546
GTACGGG48450.021.74001314
TACGGGA23400.021.6250315
Produced by FastQC (version 0.11.5)