FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841006
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14700.17479066736741472No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10050.1194997419756815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26050.041.1276861
GTGGTAT7450.041.0882531
TGGTATC7800.040.3202862
GGTATCA14600.037.2733651
TATCAAC29500.036.3048822
ATCAACG30700.034.8837243
CAACGCA31050.034.353645
TCAACGC31200.034.188484
AACGCAG32200.033.1267286
ACGCAGA35450.030.2095997
CGCAGAG35450.030.2095998
GCAGAGT38000.028.2942129
CAGAGTA29150.024.56529810-11
GCCGTCT11100.021.8248584-85
GAGTACG32700.021.638512-13
GTACGGG31900.021.51505714-15
AGAGTAC27700.021.2486210-11
CCGTCTT8100.020.9881684-85
ATTAATC3650.020.957543
GTATATC1050.004177231320.2452051
Produced by FastQC (version 0.11.5)