FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678563
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4850.053.4531022
GTGGTAT5650.045.8878971
GTATCAA20950.039.7638851
TATCAAC23250.035.8276182
TCAACGC24250.034.52294
GGTATCA11350.034.451551
CAACGCA24500.033.9971735
TATACGG500.002789322533.9971735
ATCAACG25000.033.4872133
AACGCAG25950.032.0975236
GTATATC802.3048038E-531.8770471
CGCAGAG26850.031.0216268
CTATACG550.004448548430.9065194
ACGCAGA27000.030.8492857
GCAGAGT29350.028.379249
CTATACT1203.9884617E-728.3309764
TCCTATA850.001226105124.9997622
TACTATA1251.749511E-523.7997722
TATACTG1502.7893402E-622.664785
CAGAGTA25300.022.50800910-11
Produced by FastQC (version 0.11.5)