FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678563
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10620.15650720714215188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4350.034.762522
GGTATCA11250.030.5646511
GTGGTAT5150.028.8435341
GTATCAA21400.026.4798431
TACACCG701.8058365E-423.5593345
TATCAAC25150.022.7385712
CCGTATT650.003235580621.15702240
ACGAGGT650.003238348821.15390242
AATCGGA650.003239733621.15234224
TAGTAGC650.00324666521.1445484
ATCAACG26950.020.9107153
TCAACGC26950.020.9091744
GTGCGTA804.4374025E-420.62657548
CAACGCA27350.020.6018545
ACGGGAT5900.020.51004216
ACACCGT956.0788785E-520.252766
AACGCAG28250.020.2373986
CGCAGAG28450.019.9063118
CGGGATT1802.6921043E-1019.86262917
ACGCAGA29000.019.8102577
Produced by FastQC (version 0.11.5)