FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723859
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.15016736684906867No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7820.10803208912232908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6000.046.038642
GTATCAA24200.043.7325021
GTGGTAT6700.043.137451
TATCAAC26150.040.1407622
ATCAACG27550.038.100943
TCAACGC27850.037.8431134
CAACGCA28450.037.0450135
AACGCAG29750.035.5690846
GGTATCA12200.035.535361
ACGCAGA31600.033.486727
CGCAGAG31800.033.276118
GCAGAGT33250.031.9527849
TACTATA1203.98848E-728.3314672
ACGTACG300.006808347528.33146734-35
ATTACCG1001.0558635E-425.4983225
CAGAGTA25950.024.6467410-11
AGAGTAC25150.022.05123310-11
TACCGCG1353.1732212E-522.0355877
GAGTACG29800.021.53381712-13
GTACGGG31050.020.39318314-15
Produced by FastQC (version 0.11.5)