FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642713
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12410.1930877390063683No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.11529251781121588No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC250.00779466851.000055
GTATCAA23700.041.2608151
GTGGTAT6950.038.540211
GGTATCA13150.037.505121
TGGTATC7150.036.856052
TATCAAC26650.036.6820642
ATCAACG27050.036.1368183
TCAACGC27250.035.8715934
CAACGCA27650.035.3526545
AACGCAG28350.034.4797486
ACGCAGA29400.033.2431567
CGCAGAG30600.031.9395038
GCAGAGT32150.030.5318229
CAGTCGG1159.177218E-625.8655649
CAGAGTA23700.024.83265510-11
ACCGTGT900.00171178823.6074588
GAGTACG25400.023.08697912-13
GTATTGT1703.2699427E-722.508781
GTTATAC1152.7218522E-422.1739313
AGAGTAC21850.021.29533410-11
Produced by FastQC (version 0.11.5)