FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453797
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14640.10070181737890503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6850.030.5241451
GTATCAA36200.030.4758821
TATCAAC38800.028.4160772
ATCAACG39750.027.5247083
TCAACGC39850.027.454694
GGTATCA16150.027.0860121
CAACGCA40600.027.0838725
AACGCAG41600.026.3667346
ACGCAGA42350.025.9006827
AGAGTAC41950.025.76065411
CGCAGAG42800.025.564138
TGGTATC7550.025.127752
CAGAGTA43450.024.8037810
GCAGAGT46550.023.505539
AGTACGG45650.023.49524113
GAGTACG48150.022.2174612
ACGGGAT9150.021.64050916
TTAACGG903.9927847E-521.39151235
CGGGATT2500.020.90112317
GTACGGG51150.020.80757114
Produced by FastQC (version 0.11.5)