FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154743
Sequences flagged as poor quality0
Sequence length61
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6100.031.5752851
GTATCAA24300.027.8553051
TGGTATC7100.027.4977262
GGTATCA12700.026.4323161
TATCAAC28950.023.5559082
TCAACGC30300.022.6780684
CAACGCA30450.022.5673315
ATCAACG30600.022.457683
TGCCGAC4550.022.36563355
AACGCAG32250.021.1372996
ACGCAGA33000.020.6586937
AGAGTAC31750.020.61157611
CAGAGTA32500.020.3879710
CGCAGAG33300.020.1432488
AGTACGG33450.019.48522613
TACGGGC6100.018.93696215
GTATATA1654.2446118E-818.3437371
CTGACGT750.007413587518.33261145
GCAGAGT37350.018.1085999
GAGTACG36250.017.97860312
Produced by FastQC (version 0.11.5)