FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417223
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18470.1303252910798089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44300.046.15241
GTGGTAT10450.041.489371
TATCAAC48900.041.455852
ATCAACG52000.038.9816933
TCAACGC52400.038.6841244
CAACGCA53150.038.0582965
TGGTATC11350.037.4438442
AACGCAG54550.037.159456
GGTATCA21500.035.9820481
ACGCAGA56650.035.7794347
CGCAGAG57550.035.21998
GCAGAGT61750.032.9629
CAGAGTA49900.024.9932410-11
AGAGTAC48650.023.23345610-11
GAGTACG55350.022.37875612-13
CCGTCTT13600.021.8786184-85
ACGGGAT9450.021.80922516-17
AGTACGG52050.021.55252612-13
GTACGGG58600.020.70253614-15
ACGGGAG8850.020.4068516-17
Produced by FastQC (version 0.11.5)