FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728522
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.12282169390959444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49450.043.070121
GGTATCA24750.038.990191
CAACGCA56150.037.6160435
TCAACGC56150.037.6160434
TATCAAC56600.037.5454862
GTGGTAT11700.037.4245031
ATCAACG57300.036.8610953
AACGCAG58650.036.085096
TGGTATC12000.035.772092
CGCAGAG59900.035.332068
ACGCAGA61850.034.2868277
GCAGAGT66800.031.8733529
CAGAGTA52150.025.91428810-11
GTCGTAC1401.5286987E-624.2921891
TACCGTT950.002344285122.3672647
AGAGTAC49750.022.29532810-11
GAGTACG62050.021.64271512-13
ACGGGAT11350.020.96807916-17
GTACGGG63750.020.86558714-15
AGTACGG56600.019.33424812-13
Produced by FastQC (version 0.11.5)