FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020714
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39350.028.0345421
GGTATCA19600.027.3698461
TGGTATC9300.026.3152372
TATCAAC43600.025.0382672
GTGGTAT10400.024.8649921
ATCAACG45300.024.2134533
CGATCGT801.6090204E-524.06248947
TCAACGC45650.023.9640274
CAACGCA45800.023.8255275
AACGCAG46550.023.44346
AGAGTAC47200.022.89383511
ACGCAGA48250.022.6185347
CAGAGTA49200.021.85033810
CGCAGAG50050.021.7506948
ACGATCG650.003239667321.15488415
AGTACGG51800.020.49226813
TATACCG700.004991598419.6331255
GAGTACG54900.019.43340312
GCAGAGT56850.019.295059
GTATTAG5100.018.8795531
Produced by FastQC (version 0.11.5)