FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019512
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11920.11691868266386271No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT11050.10838518820769152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39350.030.4834921
TGGTATC8450.030.267652
GTGGTAT9000.028.4291211
TATCAAC44050.027.0330122
TCAACGC45750.026.1359044
AACGCAG45900.026.0568856
CAACGCA45900.026.0517715
ATCAACG46600.025.7194253
CGCAGAG46950.025.415588
AGAGTAC45900.025.3405611
ACGCAGA47300.025.2856467
GTGCCGT550.001223330625.0109561
CAGAGTA46550.024.98426410
GCAGAGT49600.024.0576929
GGTATCA17750.023.2496221
ACGGGGC3700.022.29831316
AGTACGG51400.022.2578213
GTACGAC752.8800528E-421.9912873
GAGTACG52700.021.96753512
ACGGGAT8650.021.61948216
Produced by FastQC (version 0.11.5)