FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462423
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46650.030.9642871
GTGGTAT9100.029.932791
TGGTATC9400.028.088812
TATCAAC52400.027.7660662
TCAACGC55400.026.1488844
ATCAACG56250.025.7101523
GGTATCA20700.025.5202161
CAACGCA57300.025.2809525
AACGCAG58400.024.8535376
CAGAGTA58500.024.48714410
AGAGTAC58700.024.31086211
ACGCAGA59900.024.1402037
CGCAGAG61150.023.5576348
GCAGAGT63050.022.9801089
AGTACGG63900.021.9059613
GAGTACG66500.021.2540812
GTACGGG69400.020.09064514
TACGGGG27350.019.90985715
TACGGGC9500.019.10647415
CGGGATA3150.018.33449617
Produced by FastQC (version 0.11.5)