FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1875637
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18980.10119228827326396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47400.029.6078051
GGTATCA22150.027.9526621
GTGGTAT10650.027.6470341
TGGTATC10550.026.0637842
TATCAAC53450.026.0311152
ATCAACG54550.025.350243
CAACGCA55150.025.1694475
TCAACGC55650.024.8465084
AACGCAG56450.024.5911256
AGAGTAC55900.023.75757411
ACGCAGA59150.023.4227757
CAGAGTA59200.022.8506510
CGCAGAG60900.022.7497088
AGTACGG60400.021.71905313
GCAGAGT65550.021.4753829
GAGTACG63300.020.80814212
ACGGGAT11400.020.74816
GTACGGG68550.018.97687514
TACGGGA32000.018.47868715
TGCCGAC7200.018.33739955
Produced by FastQC (version 0.11.5)