FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36237
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3110.8582388166790849No Hit
CCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGACGGAGAGA630.17385545161023264No Hit
GTATCAACGCAGAGTACGGGCTGTCTCTTATACACATCTGACGCTGCCGACGACGGAGAGA440.12142285509286088No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGG150.00574833354.976527
ACGGGGC257.772078E-443.9812216
GTGGTAT551.8480932E-839.9829251
ACGGGGT300.001905112636.65101616
TGGTATC658.047937E-833.8317032
GGTATCA1350.030.5425111
TACGGGG952.3392204E-928.93500915
GACGGAG858.335646E-725.90708755
CGACGGA858.335646E-725.90708754
ACGACGG952.196315E-623.18002353
GTATCAA2200.021.2409291
CAGAGTA2001.8189894E-1220.61619610
AGAGTAC2001.8189894E-1220.61619611
AACGCAG2300.020.3174086
ACGCAGA2300.020.3174087
GCAGAGT2300.020.3174089
TATCAAC2300.020.3174082
CAACGCA2300.020.3174085
GACGACG1107.829683E-620.01911252
TCAACGC2350.019.8851244
Produced by FastQC (version 0.11.5)