FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649044
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48450.043.777131
GTGGTAT11100.043.270911
TGGTATC10550.041.492792
GGTATCA24700.041.128371
TATCAAC55050.038.755582
ATCAACG56050.038.060673
CAACGCA56950.037.6095665
TCAACGC57050.037.3935284
AACGCAG60500.035.543216
CGCAGAG63600.033.8775758
ACGCAGA63500.033.8629767
GCAGAGT68450.031.601369
CAGAGTA56350.025.83022110-11
GAGTACG61100.023.54321712-13
AGAGTAC52450.022.07915910-11
GTACGGG64000.022.0444814-15
CGTCAGA3500.020.6415525
CCGTCTT17100.020.50464684-85
TACTCCG4000.020.1862265
ATATCGC1708.259329E-619.9987356
Produced by FastQC (version 0.11.5)