FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574318
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9000.030.258831
GTATCAA41100.029.5828421
TGGTATC9550.028.2084032
CGGTATA400.00788233127.4940439
GGTATCA20500.025.629431
TATCAAC47750.025.6178362
TCAACGC48400.024.8154934
CAACGCA48900.024.6757345
ATCAACG49300.024.4739723
AACGCAG50100.024.0854636
AGAGTAC48650.023.85268211
CGCAGAG51000.023.554138
ACGCAGA51500.023.2713347
CAGAGTA50450.023.05468410
TGCCGAC5200.022.21590455
GCAGAGT54950.021.8651459
CTGCGTA650.003242555521.1512810
AGTACGG53650.021.06789413
GAGTACG55350.020.617612
TACGGGC9600.020.33995215
Produced by FastQC (version 0.11.5)