FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332063
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA16010.12018951055618239No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14720.11050528390924452No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA14560.10930413951892666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39400.043.0408021
TGGTATC10000.040.373342
GTGGTAT10850.038.780121
TCAACGC46100.036.503354
GGTATCA20750.036.049421
ATCAACG46800.036.0481643
TATCAAC47650.035.407782
CAACGCA47900.035.2203375
AACGCAG49100.034.532656
ACGCAGA51700.032.7125747
CGCAGAG51950.032.6369488
GCAGAGT57800.029.3337259
TACCGTC850.001227756524.9961577
CAGAGTA46400.024.268910-11
GAGTACG52350.021.30761312-13
AGAGTAC45000.021.2939510-11
CCGTCTT13100.020.76562384-85
GTATTAG5850.020.3425731
GCCGTCT17750.019.1570284-85
ACGGGAT8250.019.05767616-17
Produced by FastQC (version 0.11.5)