FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2879
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT40.13893713094824592No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGT30.10420284821118443No Hit
ATCAACGCAGAGTACGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTG30.10420284821118443No Hit
GGATAGCCTCCAGGCCAGAAAGAGAGAGTAGCGCGAGCACAGCTAAGGCC30.10420284821118443No Hit
GCGTTAAACGGGTAATGGTCGGTTTAGGCAAACCGGTCACTGTCTCTTAT30.10420284821118443No Hit
CAGCTACTAGGCAAGCCGGCTGTGTGCTCCTTATACCCTGTCTCTTATAC30.10420284821118443No Hit
GTGGTCGAACTGCTCGAGCTACCAATGAAGGCCTCAGTCTGATGCCTGTC30.10420284821118443No Hit
TCCTTAGTGAGATTTTCCGTTCTCATTCCCTGTGTCGATCCCGTACTCTG30.10420284821118443No Hit
GTTGCGGAGGAAAAGCGAAGCGAGGCGCACGAGTACGAGCGAAGTCTGGT30.10420284821118443No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCT30.10420284821118443RNA PCR Primer, Index 8 (95% over 24bp)
GAACTGGAAGCCTTGCACCAAGACTTGCCAAGTCCTGGAGCTGTCTCTTA30.10420284821118443No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC550.00485132119.3181884-85
Produced by FastQC (version 0.11.5)