FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9441
Sequences flagged as poor quality0
Sequence length61
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200.2118419658934435No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG150.005607407555.01
CTGTGTC150.005607407555.029
GTATCAA350.003898030431.4285721
AACGCAG400.007488869627.56
ACGCAGA400.007488869627.57
TCAACGC400.007488869627.54
CAGAGTA400.007488869627.510
AGAGTAC400.007488869627.511
GAGTACG400.007488869627.512
CGCAGAG400.007488869627.58
TATCAAC400.007488869627.52
ATCAACG400.007488869627.53
CAACGCA400.007488869627.55
CTGACGC1106.9184553E-620.00000255
TCTGACG1509.96026E-514.66666854
ATCTGAC1804.6817274E-412.22222253
CATCTGA1959.1838586E-411.28205152
ACATCTG2404.2837684E-410.312555
CACATCT2557.495083E-49.70588354
ACACATC2650.00106727459.339622553
Produced by FastQC (version 0.11.5)