FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123973
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA650.065.384611
GTATCAA2900.064.482761
TACACCG200.003216699263.755
TATCAAC3150.059.3650782
CTATCTT250.007779950351.04
GTCAGAT250.007779950351.06
CGTCAGA250.007779950351.05
CAACGCA3900.049.038465
AACGCAG3900.049.038466
ATCAACG3900.047.948723
CGCAGAG4000.047.81258
TCAACGC3950.047.3417744
ACGCAGA4050.047.222227
TGCTATG409.287206E-442.52
GCAGAGT4550.042.0329679
CACTATG500.002779444734.07
GCCTTGA550.00443286730.909091
CGGCCGG300.006795445528.33333228-29
TCTGTAC600.00678253328.3333323
CATACAA600.00678253328.3333322
Produced by FastQC (version 0.11.5)