FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123973
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1240.10002177893573602No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTA150.005775007454.991535
GTGGTAT654.1836756E-1142.3011781
TGGTATC751.9463187E-1036.661022
TGCCGAC701.5858313E-731.4364155
TTACTGT350.00408874831.4237348
GGTATCA1950.031.0208631
GTACTAC453.779632E-430.550856
ACGGGCA506.9861376E-427.5068616
CTGCCGA1355.456968E-1226.48808955
GTATCAA3750.025.6627141
ACGGGAA600.002022423522.92238416
TATCAAC4200.022.913142
TACTACA600.002027178222.9131377
TACGGGC852.5415802E-522.65270815
TCAACGC4550.021.7548894
ATCAACG4650.021.2870453
CGCAGAG4650.021.2870458
AACGCAG4800.020.6218246
CCCAGAA804.4161483E-420.6218222
CAACGCA4850.020.4092275
Produced by FastQC (version 0.11.5)