FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19251
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCCT200.003175526663.7500046
GGTATCA401.4049065E-553.1250041
AAGGTGG250.00768099651.05
GTATCAA708.784889E-636.4285741
TATCAAC802.1923954E-531.8750022
GCCGTCT551.7841558E-730.9090982-83
CGTCTTC551.7841558E-730.9090984-85
AACGCAG904.9003615E-528.3333366
ACGCAGA904.9003615E-528.3333367
CCGTCTT455.0286755E-528.33333684-85
TCAACGC904.9003615E-528.3333364
ATCAACG904.9003615E-528.3333363
GCAGAGT957.0830036E-526.8421069
CAACGCA957.0830036E-526.8421065
CAGAGTA907.1122486E-1025.97222310-11
CGCAGAG1001.0040944E-425.58
GAGTACG1054.2455213E-922.26190612-13
GTACGGG1054.2455213E-922.26190614-15
GTATGCC1800.020.06944784-85
AGTACGG901.296874E-518.8888912-13
Produced by FastQC (version 0.11.5)