FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19251
Sequences flagged as poor quality0
Sequence length61
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240.12466884837151317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGAC150.005667894655.07291855
GGCCATG257.6145807E-444.0583315
TACGGGG350.003975068731.47023815
ACAGCAG350.004026264431.3884963
GCTGCCG453.6312873E-430.59606654
GTATCAA653.0256142E-629.5776231
CTGCCGA506.729299E-427.53645755
AGAGTAC705.3879558E-627.46493311
GTACGGG759.024385E-625.70069314
TCAACGC759.210091E-625.633944
CAGAGTA759.210091E-625.6339410
GCAGAGT759.210091E-625.633949
CGCAGAG759.210091E-625.633948
TATCAAC759.210091E-625.633942
CGCTGCC550.001174064925.03314253
AGTACGG801.4886773E-524.09440213
AACGCAG801.5192481E-524.031826
ATCAACG801.5192481E-524.031823
CAACGCA801.5192481E-524.031825
ACGCAGA903.7767903E-521.3616167
Produced by FastQC (version 0.11.5)