FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146142
Sequences flagged as poor quality0
Sequence length61
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGT150.005782084554.979474
GAGTTGC257.835102E-443.9986320
GGGGATT257.848329E-443.98357413
TAAAAAG451.7903403E-742.77644315
TGGTATC901.8189894E-1236.6655242
GTGGTAT1200.034.409271
GTATCAA4450.031.5482731
GTCTTAG350.0040681331.4599021
AATGGAC350.004074963331.44912537
TCCAATC350.00408865631.4275932
TGCCGAC1750.028.2945255
GGTATCA1950.028.2332481
TATCAAC5000.028.0491282
TGCCAAA400.007865353527.48973311
TAACACT400.007865353527.4897334
CAACGCA5400.025.9625245
TCAACGC5450.025.7243374
ATCAACG5600.025.526183
CGGGACT550.001221655224.99922417
CAGAGTA5700.024.59607710
Produced by FastQC (version 0.11.5)