FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273559
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT2800.10235451950036373No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTAG200.00320786363.8150521
TCTATAT200.00322184563.7450643
GTATCAA8250.051.567721
TATAAGT250.007781067451.01472
CAACGCA9800.043.3639875
TATCAAC10000.042.937372
ACGCAGA10000.042.9216777
AACGCAG10000.042.9216776
TCAACGC9950.042.7102624
GACCGTG409.310486E-442.4967087
ATCAACG9900.042.4967083
CGCAGAG10050.042.2852868
GGTATCA3900.041.452511
GCAGAGT11150.038.113649
GACTAGG500.002771394334.0346951
TTCTAGG500.002781356534.0098042
AAGGTCG500.00278634833.9973685
TACACTG1201.1174052E-831.8725325
GTCCTAA550.00442002530.940631
ACTACTC550.004443826630.9066985
Produced by FastQC (version 0.11.5)