FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310336
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44950.045.1990851
TATCAAC49450.041.4249952
GTGGTAT9350.040.0039141
TGGTATC9000.039.1937942
TCAACGC52800.038.7937434
ATCAACG53650.038.179123
CAACGCA54550.037.703585
GGTATCA22900.036.935931
AACGCAG56050.036.9220126
CGCAGAG57550.035.7381448
ACGCAGA58750.035.080517
GCAGAGT61600.033.527739
CAGAGTA53050.025.83407210-11
GAGTACG59450.022.9099812-13
AGAGTAC51200.022.41002710-11
GACAGGT6100.022.2972621
GTACGGG64000.020.98282614-15
AGTACGG56800.020.20058612-13
AGACCCG1708.262052E-619.997385
CCGGTCG1100.005482696519.3163859
Produced by FastQC (version 0.11.5)