FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310336
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC8100.031.571642
GTATCAA43450.030.8345381
GTGGTAT9100.028.7197381
TATCAAC48600.027.837362
CGATCGT1004.312824E-927.5020847
GGTATCA19300.026.2276131
TCAACGC52200.025.430824
ATCAACG53050.025.1288873
AACGCAG53400.025.0680876
CAACGCA52850.025.0679575
CGCAGAG53850.024.6563078
ACGCAGA54500.024.5126237
AGAGTAC54150.024.22158611
CAGAGTA55800.023.69975310
GCAGAGT57800.023.0197549
AGTACGG60400.021.39980913
CGGGGTA1951.8189894E-1221.15625417
CCGATCG1306.999653E-821.15463846
GAGTACG62000.021.06692512
ACGGGTC2053.6379788E-1220.12424316
Produced by FastQC (version 0.11.5)