FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374754
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53940.39236110605970237No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.3127104922044235No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.19952660621463914No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA19470.1416253380604821No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC18010.1310052562131116No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT17210.12518603328304556No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT15510.11282018455665523No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA15010.10918317022536395No Hit
GTATCAACGCAGAGTACGGGATCGACACAGGGAATGAGAACGGAAAATCT14140.10285476528891714No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA14120.1027092847156655No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC13910.10118173869652317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17800.058.975022
GTGGTAT20500.051.835191
GTATCAA87800.043.0373761
GGTATCA42950.039.9812851
TATCAAC100900.037.403562
TCAACGC105200.035.95424
AACGCAG107900.035.2527286
ATCAACG107550.035.1290743
CAACGCA108000.035.0233275
ACGCAGA115000.033.001147
CGCAGAG115250.032.892688
GCAGAGT120450.031.4373749
GACAGGT4200.027.324551
CAGAGTA86800.026.58615110-11
GACAGGG3350.025.3760341
CCCATAG2053.4651748E-922.8074861
GAGTACG101250.022.66513412-13
TACGTCA1152.7239605E-422.1740265
AGAGTAC88000.022.11862810-11
GACAGAT2651.4006218E-1020.851441
Produced by FastQC (version 0.11.5)