FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662701
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11350.032.724381
TGGTATC11400.032.0778922
GTATCAA54150.029.4180321
TATCAAC59400.026.8935892
GGTATCA27750.026.1742631
ATCAACG63450.025.1724323
TCAACGC62800.025.1680564
CAACGCA63550.024.8687845
AACGCAG64650.024.4471236
AGAGTAC65000.023.94425611
ACGCAGA66900.023.625627
CGCAGAG67600.023.4223428
CAGAGTA66850.023.3192510
GCAGAGT71250.022.1472999
AGTACGG71750.021.42596613
GAGTACG73700.020.96848712
TACGGGG30650.020.81714215
GTACGGG80050.019.135714
CGTTCCG750.007411488318.33408445
ACGGGGC7700.017.8589516
Produced by FastQC (version 0.11.5)