FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371164
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52370.2208619901449246No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37710.15903581532108282No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.10227044607627309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21550.044.7714772
GTGGTAT23400.042.8747371
GTATCAA79650.042.538081
TATCAAC90150.037.6707232
ATCAACG92750.036.520763
TCAACGC93000.036.376894
CAACGCA93400.036.26665
GGTATCA42600.035.825331
AACGCAG98100.034.7023546
ACGCAGA105550.032.330097
CGCAGAG106250.032.0370988
GCAGAGT113500.030.028759
CAGAGTA79100.024.6869710-11
GAGTACG85600.022.71308312-13
AGAGTAC76150.021.76473810-11
AGTACGG82000.020.1083612-13
GTACGGG96900.019.91089614-15
CGTACCG1150.00708432518.4785355
GTACCGA1851.713738E-518.3767157
TACGGGG38750.017.71167414-15
Produced by FastQC (version 0.11.5)