FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994280
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.11031550233668291No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57400.042.579951
GTGGTAT14050.042.0520941
TGGTATC14050.040.835822
TATCAAC64100.038.1898772
ATCAACG66050.036.9309273
TCAACGC66100.036.9029924
CAACGCA66850.036.552545
GGTATCA28650.036.4972081
AACGCAG71100.034.6066936
ACGCAGA73000.033.5304877
CGCAGAG75300.032.5063178
GCAGAGT78400.031.3293959
CGACGGA1051.472274E-424.2829919
CAGAGTA64600.024.10913810-11
CGACTCT2305.4387783E-1022.1719825
GAGTACG70850.021.77243612-13
AGAGTAC60700.021.4926510-11
GTACGGA2902.1827873E-1120.5200861
GTACGGG79100.019.31405814-15
AGTACGG69100.018.91068612-13
Produced by FastQC (version 0.11.5)