FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762685
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35750.20281559098761265No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.1476157112586764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13950.045.3968282
GTATCAA61250.043.2420541
GTGGTAT15500.042.5132521
TATCAAC67800.038.8664972
TCAACGC71200.036.945584
ATCAACG72050.036.627683
GGTATCA32900.036.439931
CAACGCA73000.035.976385
AACGCAG76750.034.329316
CGCAGAG80450.032.5929228
ACGCAGA80900.032.5157627
GCAGAGT88400.029.7098439
CAGAGTA64400.025.27417810-11
CGACTCT2203.110472E-1023.1798365
GAGTACG70600.022.93424412-13
GTATTAG5550.022.2141321
AGAGTAC62250.022.08508510-11
GTACGGG78500.020.40937414-15
AGTACGG67550.020.06917412-13
CGTCAGA3850.019.8684315
Produced by FastQC (version 0.11.5)