FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762685
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18880.10710932469499657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60300.030.4723661
GTGGTAT11050.029.8721161
TGGTATC10850.029.6453512
TATCAAC65300.028.0389372
TCGCGCG606.315653E-527.50255419
GGTATCA28550.026.977331
TCAACGC68650.026.6661474
ATCAACG69050.026.4733683
CAACGCA70850.025.954515
AGAGTAC69500.025.28293411
AACGCAG73900.024.9970266
CGCAGAG73550.024.9686058
CAGAGTA71300.024.83608210
ACGCAGA75200.024.6021527
GCAGAGT79500.023.0685779
AGTACGG76400.022.6775213
GAGTACG77850.022.35921312
GCGCGAA650.003239215821.1552121
TACGGGG31300.020.73616215
TGCCGAC6500.020.73387155
Produced by FastQC (version 0.11.5)