FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830666
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49700.029.4484271
GTGGTAT10500.027.2490221
GGTATCA24350.026.776651
TATCAAC56450.025.8635522
TCAACGC57850.025.2314384
ATCAACG58000.025.1682473
CAACGCA59250.024.6829955
TGGTATC11400.024.6010112
AACGCAG59600.024.585516
AGAGTAC59100.023.95947811
ACGCAGA61400.023.8654167
CAGAGTA61400.023.37479810
TATACCG600.002040302822.9083235
CGCAGAG63650.022.8922048
AGTACGG65100.021.62807313
CCGATCG903.9967243E-521.38928246
GAGTACG67450.020.91359112
GCAGAGT70500.020.8256179
CGATCGT956.0612547E-520.2635347
GTACGGG73450.018.9446914
Produced by FastQC (version 0.11.5)