FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000489
Sequences flagged as poor quality0
Sequence length61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31500.031.2641491
TGGTATC6100.028.3912
TATCAAC34450.028.1679672
GTGGTAT6550.027.2989641
TCAACGC35800.027.0949254
ATCAACG36600.026.5777653
CAACGCA36650.026.4678545
AACGCAG38100.025.603526
ACGCAGA38750.025.17537
AGAGTAC38350.024.94751411
CGCAGAG39050.024.911528
GGTATCA13400.024.840181
CAGAGTA38800.024.7974110
GCAGAGT41250.023.5233429
AGTACGG43350.021.95200513
TACGCGA650.00324274121.15015229
GTACGCG650.003243681721.14909428
TACGGGG18250.020.6474615
GAGTACG46400.020.56317112
GTACGGG47550.019.8983414
Produced by FastQC (version 0.11.5)